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The maintenance of aseptic conditions is an absolute requirement in tissue culture labs. Autoclave , also known as steam sterilizer, is the most effective machine for the sterilization of lab equipment, water, or media. The machine uses steam under pressure to kill bacteria, viruses, and spores present in/on the equipme Autoclave : Autoclave is a large vessel through which steam is circulated for sterilization of objects. Various scientific experiments and industrial processes. Learn about autoclaves: their working principle, diagram, key uses in sterilization, and major benefits in medical, lab, and industrial settings. An autoclave is a machine used to carry out industrial and scientific processes requiring elevated temperature and pressure in relation to ambient pressure and temperature. Autoclaves are found in many medical settings, laboratories, and other places that need to ensure the sterility of an object.