Chromatography Definition, stationary phase, mobile phase. Different types of chromatography with their definition, steps, uses and examples. • Chromatography is a method of physical separation in which components of mixture gets separated on two phases. • One of the phase is the immobile phase is called stationary phase and the other phase is the mobile fluid that flows over the stationary phase. • During the movement of the sample, a separated result is formed by the repeated desorption and sorption in the direction of the mobile phase migration. Chromatography is a method used to separate the individual components of a mixture on the basis of their interactions with a stationary and a mobile phase. Each component interacts differently with the two phases which causes them to move at different speeds and separate. Chromatography is a method of physical separation in which components of mixture gets separated on two phases. One of the phase is the immobile porous bed bulk liquid which is called stationary phase and the other phase is the mobile fluid that flows over the stationary phase under gravity.
