Introduction Gel electrophoresis is the standard lab procedure for separating DNA by size (e.g., length in base pairs) for visualization and purification. Electrophoresis uses an electrical field to move the negatively charged DNA through an agarose gel matrix toward a positive electrode. Shorter DNA fragments migrate through the gel more quickly than longer ones. Thus, you can determine the approximate length of a DNA fragment by running it on an agarose gel alongside a DNA ladder (a ... Agarose Gel Electrophoresis: As described earlier, agarose gel electrophoresis is used to separate DNA fragments based on their size. It is commonly used for DNA analysis, such as genotyping, DNA sequencing, and restriction enzyme digestion. Learn how to separate DNA fragments by size using agarose gel electrophoresis, a common technique in molecular biology. Find out the factors affecting the migration of DNA, the steps of sample preparation and loading, and the methods of visualization and estimation. In this article, we will consider how agarose gel electrophoresis works, how it can be interpreted and some of its purposes with emphasis of its use for DNA separation.
