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The biuret test is a colorimetric assay that detects the presence of at least two peptide bonds in a molecule, such as proteins. It involves a copper (II) ion forming a mauve complex with peptides in an alkaline solution, and can be modified by BCA or Lowry reagents for higher sensitivity. Biuret is a compound formed by heating urea at 180 0 It is the result of the condensation of 2 molecules of urea. The peptide bonds in Biuret give a positive result for the test hence the reagent is named so. Biuret test is a general test for compounds (proteins and peptides) having two or more peptide (CO-NH) bonds. Histidine is the only amino acid that gives Biuret test positive. Principle: When biuret is treated with dilute copper sulfate in alkaline medium, a purple colored compound is ... Biuret test is used for the detection of peptide bonds in the protein. This test is not suitable for amino acid only. The protein solution when reacts with CuSO4 in alkali condition (NaOH/KOH), purple to violet-colored copper (II) cation complex is formed. Biuret reagent is essential in biochemical analysis, serving a key role in both qualitative and quantitative protein detection. This comprehensive guide explores its composition, preparation, underlying principles, applications, and significance of the Biuret test, a vital technique used in laboratories globally.
