Flow cytometry: Is a technology that rapidly analyzes single

Flow cytometry is a technology that rapidly analyzes single cells or particles as they flow past single or multiple lasers while suspended in a buffered salt-based solution. Each particle is analyzed for visible light scatter and one or multiple fluorescence parameters. Flow cytometry is a technique that enables the measurement of properties of individual particles (cells) in a heterologous population. This allows researchers to phenotype each cell based on these properties and, in some cases, to sort cells into different subpopulations (fluorescence-activated cell sorting or FACS). In a flow cytometer, cells in suspension pass through a laser in a single file, which enables the measurement of particle size and granularity. Further phenotyping occurs by ... Flow cytometry is a laser-based lab test that can detect chemical and physical differences of cells or particles. Healthcare providers commonly use it to evaluate bone marrow, peripheral blood and other fluids in your body. Explore our flow cytometry guide to uncover flow cytometry basics, traditional flow cytometer components, key flow cytometry protocol steps, and proper controls.