What is Paper Chromatography ? Paper chromatography is a chromatography technique in which a solution is forced to flow through paper sheets or strips as the adsorbent and stationary phase. In analytical chemistry, paper chromatography is a technique used to separate dissolved chemical substances by taking advantage of their different rates of migration across paper sheets. In this procedure, chromatography paper serves as the stationary phase and is suspended in a mixture of solvents, which ... Paper Chromatography - Introduction, Types, Principle, Instrumentation, Steps, Rf values, Applications, Advantages and Limitations. Paper Chromatography . Chromatography is used to separate mixtures of substances into their components. All forms of chromatography work on the same principle. They all have a stationary phase (a solid, or a liquid supported on a solid) and a mobile phase (a liquid or a gas). The mobile phase flows through the stationary phase and carries the components of the mixture with it. Different components travel at different rates. We'll look at the reasons for this further down the page. In paper chromatography , the ... Paper chromatography - Principle, Procedure, types and applications Principle of paper chromatography : This technique is a type of partition chromatography in which the substances are distributed between two liquids, i.e., one is the stationary liquid (usually water) which is held in the fibers of the paper and called the stationary phase, the other is the moving liquid is the moving liquid or developing solvent and called the moving phase. Cellulose filter paper is often used as the ...