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Learn how to read a Widal test report with this step-by-step guide. Understand the significance of titers, Salmonella antigens (O, H, AH, BH), and what results indicate typhoid infection. The Widal test, developed in 1896 and named after its inventor, Georges-Fernand Widal, is an indirect agglutination test for enteric fever or undulant fever whereby bacteria causing typhoid fever are mixed with a serum containing specific antibodies obtained from an infected individual. Widal test: Introduction, Principle, Procedure, Result interpretation, Applications and limitations Introduction Widal test is a serological test which is used for the diagnosis of enteric fever or typhoid fever. The test was developed by Greembaum and Widal in 1896. Typhoid or enteric fever is caused by a gram negative bacteria Salmonella enterica (Salmonella Typhi or Salmonella Paratyphi), found in the intestine of man. Salmonella paratyphi also causes Typhoid but of a milder form ... A positive Widal test means Salmonella Typhi antibodies are present in the blood which is an indication of active typhoid. And if the test results are negative it means there is no active infection.